These tracks show the signals and estimated expression levels for RNA-seq from several multilineage progenitor hematopoietic cell types and lymphoid cell types. These data are unstranded. They were published in a paper from the Amit laboratory, Lara-Astiaso et al (2014). The data are provided here to complement the other RNA-seq data from the VISION project, which did not cover lymphoid cell types.
These data that are non-directional, so the signal from both strands is shown in the data tracks.
The track names give an abbreviation for the blood cell type or cell line. Mouse primary blood cells purified predominantly using cell surface markers include: LSK = Lin-Sca1+Kit+ cells from mouse bone marrow containing hematopoietic stem and progenitor cells, CMP = common myeloid progenitor cell, MEP = megakaryocyte-erythrocyte progenitor cell, CLP = common lymphoid progenitor cell, B = B cell, NK = natural killer cell, T_CD4 = CD4+ T cell, and T_CD8 = CD8+ T cell.
The methods for isolating RNA and preparing the libraries for sequencing are described in Lara-Astiaso et al. (2014).
Belinda Giardine generated the tracks displayed and developed the track hub.
Lara-Astiaso D, Weiner A, Lorenzo-Vivas E, Zaretsky I, Jaitin DA, David E, Keren-Shaul H, Mildner A, Winter D, Jung S, Friedman N, Amit I. Immunogenetics. Chromatin state dynamics during blood formation. Science. 2014 Aug 22;345(6199):943-9. doi: 10.1126/science.1256271. Epub 2014 Aug 7. PMID: 25103404; PMCID: PMC4412442.
These data are available for use without restrictions.
Ross Hardison rch8@psu.edu